Inducible nitric oxide synthase-derived extracellular nitric oxide flux regulates proinflammatory responses at the single cell level

GAPDH regulates cellular heme insertion into inducible nitric oxide synthase.

Heme proteins play essential roles in biology, but little is known about heme transport inside mammalian cells or how heme is inserted into soluble proteins. We recently found that nitric oxide (NO) blocks cells from inserting heme into several proteins, including cytochrome P450s, hemoglobin, NO synthases, and catalase. This finding led us to explore the basis for NO inhibition and to identify...

Inducible nitric oxide synthase and inflammatory diseases.

Nitric oxide (NO) is a colorless gas at room temperature and one of the simplest molecules known, yet it has been implicated in a wide variety of regulatory mechanisms ranging from vasodilatation and blood pressure control to neurotransmission. It is also involved in nonspecific immunity and participates in the complex mechanism of tissue injury as a major mediator of inflammatory processes and...

Inducible Nitric Oxide Synthase: What Difference

Fire sweeps through the brush. In its aftermath, dormant seeds of chaparral, savanna, heath, and scrub begin to germinate in response to a “go” signal in the smoke. Even though smokesoaked water kills the seeds, in diluted form it triggers their development. The chemical cues are nitrogen oxides (1). This lesson of death and life in the field mirrors comparable events within us, where reactive ...

Inducible nitric oxide synthase and blood pressure.

In the present studies, the influence of inducible nitric oxide synthase (NOS) inhibition with aminoguanidine on renal function and blood pressure was examined in rats. Intravenous aminoguanidine infusion (60 mg x kg-1 x hr-1) for 40 minutes to anesthetized Sprague-Dawley rats (n=7) resulted in no significant changes in mean arterial pressure or renal cortical blood flow, while medullary blood ...

Inducible Nitric Oxide Synthase-Deficient Mice